What are the advantages and disadvantages of the serial dilution - agar plate procedure? Advantages and disadvantages of spread plate technique?!? 2017-8-3  Pour Plate Method_ Principle, Procedure, Uses, And. (Follow serial dilution technique). And (Dis) Advantages ­ microbeonline. Exp 20 Micro Lab Manual - w. Advantages of the serial dilution—agar plate technique are as. What are the advantages and disadvantages of the serial dilution.

DILUTION PLATING Objective This treatment is utilized to identify the number of viable micro-organisms in a fixed amount of a liquid. It can furthermore be pretty easily modified to provide outcomes with strong substances, at the.g.

Macerated food items. Background Serial dilution entails repeatedly mixing up known amounts of supply tradition with (sterilised) water. 1 ml added to 9 ml provides a 10-collapse dilution; 1 ml added to 99ml provides a 100-flip dilution. When set quantities of this dilution series are mixed with an appropriate agar and incubated, then different quantities of colonies will become obtained. By operating back again from an quickly counted plate and using the suitable dilution aspect, the quantity of micro-órganisms in the original source tradition can be calculated. Procedure For this workout, fungus suspensions, ('clean' or 'boring') milk products, or water may be used. Place away and content label the tubes and (empty) Petri dishes as shown in the diágram below.

Each member of the group can take it in changes to perform the repeated areas below, and quick others as needed. Fire and release the lids of tubes 0 and 1. Using a sterile pipette Dealt with ASEPTICALLY move 1 ml of liquid from pipe 0 to plate 0, and USING THE SAME PIPETTE, move 1 ml of liquid from the supply lifestyle (tube 0) to tubing 1.

Then: DISCARD THE PIPETTE. Flame the advantage of tube 1.

Close off and mix the items gently. Do it again the process with the next pipe and plate: Flame and release the lids of pipes 1 and 2.

Move 1 ml of liquid from pipe 1 to plate -1, and also into pipe 2. Throw away THIS PIPETTE. Fire the advantage of tube 2. Seal and combine the items gently. Repeat the exact same measures, 5 or 6 periods, relocating along the string as proven in the movement chart below. At the end of this procedure: Take a container of sterilised ágar from the 45 °Chemical waterbath, where it provides been held just above setting temperature.

Dry the outdoors of the bottle, and flame the best and neck of the guitar area. After that Function QUICKLY AND ASEPTICALLY: Opening each Petri meal lid just slightly, pour nutrient agar into the dilution liquid already in the Petri dish, until it addresses about twó thirds of thé region - although this is usually not essential. Mix the ágar with the diIution liquid by a gentle swirling motion, then flame the mouth of the container and shift on to put another Petri meal.

When the bottle is vacant, wash it out with popular water. Keep the meals undisturbed Smooth ON THE Table to set - at minimum 10 a few minutes. Check the labelling. Seal off and invert the Petri dishes, and location them in thé incubator at án appropriate temperature.

Results After an appropriate period: Examine each Petri meal WITHOUT OPENING It all and look for individual colonies. Some will have got even more colonies than you can rely. Some may have none. Several intermediate types will become countable. Count number AND RECORD these in a table, jointly with the appropriate dilution elements.